Recombinant Anti-SNAP25 antibody [EP3274] KO Tested (ab108990) (2024)

Table of Contents
Product price, shipping and contact information Abpromise Overview Product name Description Host species Tested applications Species reactivity Immunogen Positive control General notes Properties Form Storage instructions Storage buffer Purity Clonality Clone number Isotype Research areas Associated products Alternative Versions Isotype control Positive Controls Recombinant Protein Applications The Abpromise guarantee Target Function Tissue specificity Sequence similarities Post-translationalmodifications Cellular localization Database links Alternative names Images Protocols Datasheets and documents SDS download Datasheet download References (10) Customer reviews and Q&As

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Recombinant Anti-SNAP25 antibody [EP3274] KO Tested (ab108990) (1)

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Overview

  • Product name

    Anti-SNAP25 antibody [EP3274]
    See all SNAP25 primary antibodies

  • Description

    Rabbit monoclonal [EP3274] to SNAP25

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Fetal brain, HeLa, SH-SY5Y, U87-MG, fetal hippocampus, fetal cerebellum, and Neuro-2a cell lysates; Human brain tissue; Human cerebellum tissue, mouse and rat brain tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production

    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant

  • Recombinant Anti-SNAP25 antibody [EP3274] KO Tested (ab108990) (2)Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP3274

  • Isotype

    IgG

  • Research areas

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-SNAP25 antibody [EP3274] (ab207366)
    • Alexa Fluor® 488 Anti-SNAP25 antibody [EP3274] (ab208379)
    • Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)

  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

  • Positive Controls

    • HeLa membrane extract lysate (ab29547)

  • Recombinant Protein

    • Recombinant Human SNAP25 protein (ab74529)
See Also
Glutamine Synthetase Antibody (OTI1F4) [Janelia Fluor® 669]Glutamine Synthetase Antibody (GLUL/6600) [Janelia Fluor® 525]Centrin 1 Antibody [Janelia Fluor® 669]alpha-Synuclein [p Ser129, Cys Glu134] Antibody (J18) [Janelia Fluor® 525]

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB

1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
IHC-P (2)

1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
Notes

WB
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).

IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

Application notes

Is unsuitable for Flow Cyt or IP.

Target

  • Function

    t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.

  • Tissue specificity

    Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

  • Sequence similarities

    Belongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil hom*ology domains.

  • Post-translational
    modifications

    Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.

  • Cellular localization

    Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.

  • Information by UniProt

  • Database links

  • Alternative names

    • bA416N4.2 antibody
    • Bdr antibody
    • CMS18 antibody

    see all

Images

  • Western blot - Anti-SNAP25 antibody [EP3274] (ab108990)

    All lanes : Anti-SNAP25 antibody [EP3274] (ab108990) at 1/1000 dilution

    Lane 1 : Wild-type SH-SY5Y cell lysate
    Lane 2 : SNAP25 knockout SH-SY5Y cell lysate
    Lane 3 : Neuro-2a cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 27 kDa why is the actual band size different from the predicted?

    False colour image of Western blot: Anti-SNAP25 antibody [EP3274] staining at 1/1000 dilution, shown in green; loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) staining at 1/20000 dilution, shown in red. In Western blot, ab108990 was shown to bind specifically to SNAP25. A band was observed at 27 kDa in wild-type SH-SY5Y cell lysates with no signal observed at this size in SNAP25 knockout cell line ab280041 (knockout cell lysate ab280100). To generate this image, wild-type and SNAP25 knockout SH-SY5Y cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

  • See Also
    Calcitonin Antibody (CALCA/3310) [Janelia Fluor® 585]

    Western blot - Anti-SNAP25 antibody [EP3274] (ab108990)

    All lanes : Anti-SNAP25 antibody [EP3274] (ab108990) at 1/1000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : U87-MG cell lysate
    Lane 5 : Fetal hippocampus lysate
    Lane 6 : Fetal cerebellum lysate
    Lane 7 : Neuro-2a cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] (ab108990)

    ab108990, at 1/100 dilution, staining SNAP25 in paraffin-embedded Human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-SNAP25 antibody [EP3274] (ab108990)

    All lanes : Anti-SNAP25 antibody [EP3274] (ab108990) at 1/2000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat heart tissue lysate
    Lane 7 : Rat kidney tissue lysate
    Lane 8 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 23 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?

    Blocking/Diluting buffer and concentration: 5% NFDM /TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] (ab108990)

    ab108990, at 1/100 dilution, staining SNAP25 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] (ab108990)

    ab108990 showing negative staining in human Normal colon tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] (ab108990)

    ab108990 showing negative staining in human Normal spleen tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] (ab108990)

    ab108990 showing negative staining in human Skeletal muscle tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-SNAP25 antibody [EP3274] (ab108990)

Datasheets and documents

  • SDS download

    Recombinant Anti-SNAP25 antibody [EP3274] KO Tested (ab108990) (12)

  • Datasheet download

    Download

References (10)

Publishing research using ab108990? Please let us know so that we can cite the reference in this datasheet.

ab108990 has been referenced in 10 publications.

  • Tian J et al. Dissection of the long-range projections of specific neurons at the synaptic level in the whole mouse brain. Proc Natl Acad Sci U S A 119:e2202536119 (2022). PubMed: 36161898
  • Sadeghi L et al. Proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy: potential targets in calcium regulatory network. Sci Rep 11:8252 (2021). PubMed: 33859251
  • Lee SH et al. APP Family Regulates Neuronal Excitability and Synaptic Plasticity but Not Neuronal Survival. Neuron 108:676-690.e8 (2020). PubMed: 32891188
  • Liao Y et al. d-Glucuronolactone attenuates para-xylene-induced defects in neuronal development and plasticity in Xenopus tectum in vivo. Toxicology 430:152341 (2020). PubMed: 31811891
  • Kang J & Shen J Cell-autonomous role of Presenilin in age-dependent survival of cortical interneurons. Mol Neurodegener 15:72 (2020). PubMed: 33302995
  • Shi TY et al. Kainate receptor mediated presynaptic LTP in agranular insular cortex contributes to fear and anxiety in mice. Neuropharmacology 128:388-400 (2018). WB . PubMed: 29104074
  • Almandoz-Gil L et al. In Situ Proximity Ligation Assay Reveals Co-Localization of Alpha-Synuclein and SNARE Proteins in Murine Primary Neurons. Front Neurol 9:180 (2018). Mouse . PubMed: 29623065
  • Geerts CJ et al. Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains. PLoS One 12:e0180912 (2017). PubMed: 28746398
  • Jin WS et al. Peritoneal dialysis reduces amyloid-beta plasma levels in humans and attenuates Alzheimer-associated phenotypes in an APP/PS1 mouse model. Acta Neuropathol 134:207-220 (2017). PubMed: 28477083
  • Lue LF et al. TREM2 Protein Expression Changes Correlate with Alzheimer's Disease Neurodegenerative Pathologies in Post-Mortem Temporal Cortices. Brain Pathol 25:469-80 (2015). IHC-FrFl ; Human . PubMed: 25186950

Customer reviews and Q&As

Recombinant Anti-SNAP25 antibody [EP3274] KO Tested (ab108990) (15)

Recombinant Anti-SNAP25 antibody [EP3274] KO Tested (ab108990) (2024)
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