概述
-
产品名称
Anti-Neurofilament heavy polypeptide抗体
参阅全部 Neurofilament heavy polypeptide 一抗 -
描述
鸡多克隆抗体to Neurofilament heavy polypeptide
-
宿主
Chicken
-
经测试应用
适用于: ICC, IHC-FrFl, WBmore details
-
种属反应性
与反应: Mouse, Rat, Cow, Human
-
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid
-
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze.
-
存储溶液
Preservative: 0.032% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
IgY fraction
-
纯化说明
This is an IgY prep, similar to a mammalian ammonium sulphate IgG prep from serum. It is virtually pure IgY and we estimate that about 5% of material is specific to the immunogen.
-
克隆
多克隆
-
同种型
IgY
-
研究领域
- Neuroscience
- Neurology process
- Neurodegenerative disease
- Alzheimer's disease
- Other
- Neuroscience
- Cell Adhesion Proteins
- Cytoskeletal Proteins
- Intermediate Filaments
- Signal Transduction
- Cytoskeleton / ECM
- Cytoskeleton
- Intermediate Filaments
- Class IV
- Neurofilaments
- Neuroscience
- Neurology process
- Neurodegenerative disease
- Other
- Neuroscience
- Cell Type Marker
- Neuron marker
- Axon marker
- Neuroscience
- Development
相关产品
-
Isotype control
- Chicken IgY - Isotype Control (ab50579)
-
Recombinant Protein
- Recombinant Human Neurofilament heavy polypeptide protein (ab158986)
-
Related Products
- Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab4680于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | (1) | Use at an assay dependent concentration. |
| IHC-FrFl | (1) | Use at an assay dependent concentration. |
| WB | 1/20000. Predicted molecular weight: 200-220 kDa. |
| 说明 |
|---|
| ICC |
| IHC-FrFl |
| WB |
靶标
-
功能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
-
疾病相关
Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
-
序列相似性
Belongs to the intermediate filament family.
-
翻译后修饰
There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
-
数据库链接
- Entrez Gene: 528842 Cow
- Entrez Gene: 4744 Human
- Entrez Gene: 380684 Mouse
- Entrez Gene: 24587 Rat
- Omim: 162230 Human
- SwissProt: P12036 Human
- SwissProt: P19246 Mouse
- SwissProt: P16884 Rat
see all
-
别名
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
see all
图片
-
Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab4680)
Immunohistological analysis of a rat cerebellum section stained with ab4680 at a dilution 1:5,000 in red, and co-stained with rabbit pAb to GFAP at adilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA.
Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free floating sections were stained.
-
Western blot - Anti-Neurofilament heavy polypeptide antibody (ab4680)
All lanes : Anti-Neurofilament heavy polypeptide antibody (ab4680) at 1/20000 dilution
Lane 1 : Rat spinal cord lysate
Lane 2 : Mouse spinal cord lysate
Lane 3 : Cow spinal cord lysatePredicted band size: 200-220 kDa
-
Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
Mixed neuron/glial cultures stained with a mouse monoclonal antibody to neurofilament subunit NF-L (green) and ab4680, chicken antibody to neurofilament heavy polypeptide. This antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to the NF-L antibody which stains both axonal and dendritic/perikaryal neurofilaments. The NF-L antibody therefore reveals a prominent cell body in green, while the surrounding axonal profiles are orange, since the are bound by both NF-L and the chicken NF-H antibody. Blue is a DNA stain.
-
Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody (ab4680)
Human neuroblastoma SH-SY5Y cells stained with chicken anti-NF-H (red) and mouse monoclonal to fibrillarin 38F3 (green). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000. Cultures were processed using our standard fixation and staining procedure (in protocol section).
-
Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody (ab4680)
Rat Neurons stained with chicken anti-NF-H (green). The NF-H antibody was used at a dilution of 1/100000 using our standard fixation and staining procedure (in protocol section).
-
Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)Image from Wirt SE et al, J Cell Biol. 2010 Nov 15;191(4):809-25. Epub 2010 Nov 8, Fig 4. DOI 10.1083/jcb.201003048
ab4680 staining Neurofilament heavy polypeptide (green) in murine embryoid bodies by Immunocytochemistry/ Immunofluorescence. Nuclei were visualized with DAPI.
数据表及文件
-
SDS download
-
Datasheet download
Download
文献 (135)
发表研究结果有使用 ab4680？请让我们知道，以便我们可以引用本数据表中的参考文章。
ab4680 被引用在 135 文献中.
- Laraba L et al. Inhibition of YAP/TAZ-driven TEAD activity prevents growth of NF2-null schwannoma and meningioma. Brain 146:1697-1713 (2023). IF ; Human . PubMed: 36148553
- Pei F et al. Sensory nerve niche regulates mesenchymal stem cell homeostasis via FGF/mTOR/autophagy axis. Nat Commun 14:344 (2023). PubMed: 36670126
- Al-Arbeed TA et al. Neuroregeneration of injured peripheral nerve by fraction B of catfish epidermal secretions through the reversal of the apoptotic pathway and DNA damage. Front Pharmacol 14:1085314 (2023). PubMed: 36726586
- Massih B et al. A 3D cell culture system for bioengineering human neuromuscular junctions to model ALS. Front Cell Dev Biol 11:996952 (2023). PubMed: 36866276
- Xu Y et al. Lactate promotes neuronal differentiation of SH-SY5Y cells by lactate-responsive gene sets through NDRG3-dependent and -independent manners. J Biol Chem 299:104802 (2023). PubMed: 37172727
View all Publications for this product
